Urso, Elena, Le Pera, Maria, Bossio, Sabrina, Sprovieri, Teresa, Qualtieri, Antonio

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) has been applied to the analysis of a wide range of biomolecules. To date, there are two specific areas of application where MALDI-TOF-MS is viewed as impractical: analysis of low-mass analytes and relative quantitative applications. However, these limitations can be overcome and quantification can be routine. Increased levels […]

Tian, Hui-jun, Zhang, Jia-tang, Lang, Sen-yang, Wang, Xiang-qing

MRI has had an important role in the diagnosis of Creutzfeldt-Jakob disease (CJD). The aim of our study was to compare the efficacy of different MRI sequences among six biopsy-proven patients with sporadic CJD (sCJD) and seven patients with probable sCJD. These 13 patients with CJD aged from 36 years to 75 years (mean age: […]

Stoeck, K., Sanchez-Juan, P., Gawinecka, J., Green, A., Ladogana, A., Pocchiari, M., Sanchez-Valle, R., Mitrova, E., Sklaviadis, T., Kulczycki, J., Slivarichova, D., Saiz, A., Calero, M., Knight, R., Aguzzi, A., Laplanche, J.-L., Peoc'h, K., Schelzke, G., Karch, A., van Duijn, C. M., Zerr, I.

To date, cerebrospinal fluid analysis, particularly protein 14-3-3 testing, presents an important approach in the identification of Creutzfeldt-Jakob disease cases. However, one special point of criticism of 14-3-3 testing is the specificity in the differential diagnosis of rapid dementia. The constant observation of increased cerebrospinal fluid referrals in the national surveillance centres over the last […]

Steinacker, Petra, Klafki, Hans, Lehnert, Stefan, Jesse, Sarah, Arnim, Christine A. F. V., Tumani, Hayrettin, Pabst, Alice, Kretzschmar, Hans A., Wiltfang, Jens, Otto, Markus

The clinical diagnosis of Creutzfeldt-Jakob disease (CJD) can be supported by several biochemical markers in cerebrospinal fluid (CSF) such as 14-3-3 proteins and tau protein. Unfortunately, none of the currently known markers are suited for screening or seems to be directly related to the pathophysiological process. A marker fulfilling these criteria might facilitate the early […]

Skillbäck, Tobias, Rosén, Christoffer, Asztely, Fredrik, Mattsson, Niklas, Blennow, Kaj, Zetterberg, Henrik

Importance: Identifying a clinical distinction between the invariably lethal prion disease Creutzfeldt-Jakob disease (CJD) and nonprion rapidly progressive dementias is important and sometimes difficult; thus, reliable tools for diagnosis are in great demand.; Objective: To test the diagnostic performance of dementia cerebrospinal fluid (CSF) biomarkers total tau (T-tau), phosphorylated tau (P-tau), and the T-tau to […]